Canny algorithm is used in the segmentation of the cell image and it can obtain better effect of segmentation by setting the appropriate parameters.After the histogram of cell image is analyzed,the Ostu algorithm can implement the two threshold's adaptive setting in Canny algorithm. From the test's result, it can be seen that the Otsu algorithm can solve the threshold's setting in Canny algorithm and it can improve the the effect of cell image's segmentation and the adaptive ability of Canny algorithm.