An acceleration measurement scheme with atom interference in optical waveguide at horizontal direction was presented. The optical structure, radical preparation and optical waveguide adiabatic loading were described in detail. In order to verify the measurement scheme by using the experiment means, the radical ⁸⁷Rb was cooled into Bose-Einstein condensate, then the splitting and combining of the ultracold radical was achieved by the Bragg diffraction and the process of atom interference was completed. The experiment results show that the interferometer can measure the axial acceleration of the optical waveguide, which is of reference value for the future study of multi-axis acceleration measurement.