Large-scale space development project (LSDP) is a kind of project which possesses some special characteristics and is significant for the social economy development and national security. Risk analysis is an important guarantee to accomplish these projects designed with high quality and low cost. According to the characters, management process and risk factors of the LSDP, using the theory and technology of Multi-Agent and Disturb Project Management, a new risk analysis method of LSDP based on Multi-Agent technology is proposed. It is applied to a large-scale space development project. The risk level of the project is evaluated and the key risk factors are identified. These results can provide decision information for the project risk control and management.