The single channel method of anti-navigation frequency interference mainly utilizes the prior knowledge of the interference in observed data received by the large antenna for interference mitigation, the performance of the interference mitigation is weakened in the case of the relatively lower interference-to-noise ratio in the observed data. A channel-assisted interference mitigation method for radio astronomy by parametric estimation and subtraction is proposed. To achieve the purpose of eliminating the interference signal, the proposed method utilizes the observational data received by auxiliary antenna having a higher interference-to-noise ratio to establish the estimation model of interference parameters, at the same time by constructing the difference parameters estimation model of the main and auxiliary channel to correct the estimation model. Simulation results indicate that, compared with the single channel method, the proposed method is more broadly applicable to eliminate the interference in the lower interference-to-noise ratio conditions for radio astronomy.