Based on the temperature correction model of meteorological sounding rocket, through error analysis theory, research on the method of temperature correction and uncertainty evaluation was conducted. According to the law of atmospheric density change during the falling process of rocketsonde, the mathematical model of temperature correction was established, and the formula of temperature correction was deduced. According to the error theory, eight error factors which affect the temperature correction were analyzed, and the expression of error in temperature correction was given one by one. Taking measured data as an example, using the above formula, the uncertainty of temperature inversion of sounding rocket was analyzed and calculated. The results show that the uncertainty of temperature inversion is larger in 50~60 km, and the maximum is 3.6 K; uncertainty in 40~50 km is 0.3~0.9 K; uncertainty in less than 40 km is no more than 0.3 K.The main factors that affect the uncertainty of temperature are aerodynamic heating correction, lag effect correction, structural heat conduction correction, and sensor correction for environmental heat radiation. It is not enough to use the reference atmosphere or the standard atmosphere for only single correction in data processing. Iterative correction is needed, and the maximum difference between iterative correction results and single correction results is 5.6 K.