To clarify the optimal interference parameters of microwave pulses to navigation receivers, by analyzing theoretically the tracking loop correlation processing of navigation receiver under microwave pulse interference, a interference classification method was proposed on the basis of the relationship between microwave pulse spectral lines, spread spectrum code spectral lines and coherent integration time, the correlation output power and carrier-to-noise ratio under microwave pulse interference with different parameters was further derived, finally experimental verification was carried out. Theoretical analysis and experimental results show that when the microwave pulse width is greater than or equal to the spreading code period and less than the coherent integration time, a spreading code spectral line is interfered with multiple microwave pulse spectral lines which enters the bandwidth of the coherent integrator, the microwave pulse has the best interference effect on navigation receivers, and is better than continuous wave interference; with the increase of the number of interference spectral lines, the correlation output power is increased, the carrier-to-noise ratio is decreased, and the interference effect is enhanced.