A transformation-competent artificial chromosome (TAC) library was constructed from the genomic DNA of orazy.mimuta. This library is composed of 5,000 clones while the average insertion fragment size is 45 kb. Stability test indicated that genome DNA of Orazy.mimuta was stably existence in the pYLTAC27 carrier. A probe was designed according to the sequences of cloned resistance gene. With the colony hybridization method, the genome library was screened. The result shows that resistance gene can be screened in this library.