| 引用本文: | 王正华,曹筑荣,曹孟良.小粒野生稻可转化人工染色体文库初步构建及筛选.[J].国防科技大学学报,2008,30(1):120-124.[点击复制] |
| WANG Zhenghua,CAO Zhurong,CAO Mengliang.The Construction and Screening of Genomic Library Based on Transformable Artificial Chromosome (TAC) Vector in Orazy.Minuta[J].Journal of National University of Defense Technology,2008,30(1):120-124[点击复制] |
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| 小粒野生稻可转化人工染色体文库初步构建及筛选 |
| 王正华1, 曹筑荣1, 曹孟良2 |
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(1.国防科技大学 计算机学院，湖南 长沙 410073;2.国家杂交水稻工程技术研究中心，湖南 长沙 410125)
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| 摘要: |
| 以小粒野生稻核DNA为材料构建了一个可转化人工染色体文库，获得了5000个克隆，平均插入片段约45 kb。稳定性检测结果表明，小粒野生稻基因组DNA能够在TAC载体中稳定存在。基于已克隆抗性基因的保守序列设计探针，利用菌落杂交的方法，对文库进行筛选。结果表明，该文库可以用于抗性基因的筛选。 |
| 关键词: 可转化人工染色体 基因组文库 小粒野生稻 |
| DOI： |
| 投稿日期：2007-08-14 |
| 基金项目:中国博士后基金资助项目(2005037696)；湖南省级重点实验室科研资助项目(05FJ4035) |
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| The Construction and Screening of Genomic Library Based on Transformable Artificial Chromosome (TAC) Vector in Orazy.Minuta |
| WANG Zhenghua1, CAO Zhurong1, CAO Mengliang2 |
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(1.College of Computer, National Univ. of Defense Technology，Changsha 410073，China;2.National Hybrid Rice R & D Center, Changsha 410125，China)
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| Abstract: |
| A transformation-competent artificial chromosome (TAC) library was constructed from the genomic DNA of orazy.mimuta. This library is composed of 5,000 clones while the average insertion fragment size is 45 kb. Stability test indicated that genome DNA of Orazy.mimuta was stably existence in the pYLTAC27 carrier. A probe was designed according to the sequences of cloned resistance gene. With the colony hybridization method, the genome library was screened. The result shows that resistance gene can be screened in this library. |
| Keywords: transformation-competent artificial chromosome genomic library orazy.minuta |
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