| 引用本文: | 王正华,杲修杰,曹孟良.东乡野生稻NBS-LRR类抗性基因同源序列的分离与鉴定.[J].国防科技大学学报,2008,30(2):56-60.[点击复制] |
| WANG Zhenghua,GAO Xiujie,CAO Mengliang.Isolation and Characterization of NBS-LRR Resistance Gene Homology Sequences from Dongxiang Wild Rice[J].Journal of National University of Defense Technology,2008,30(2):56-60[点击复制] |
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| 东乡野生稻NBS-LRR类抗性基因同源序列的分离与鉴定 |
| 王正华1, 杲修杰1, 曹孟良2 |
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(1.国防科技大学 计算机学院，湖南 长沙 410073;2.国家杂交水稻工程技术研究中心，湖南 长沙 410125)
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| 摘要: |
| 基于植物的抗性基因具有保守序列，根据已克隆基因的保守序列设计探针，利用菌落杂交的方法，对东乡野生稻大片段基因组文库进行筛选，得到两个候选克隆。序列比对发现其中含有NB-ARC的保守序列，可用于后续转基因鉴定。 |
| 关键词: NBS-LRR 抗性基因 菌落杂交 基因组文库 |
| DOI： |
| 投稿日期：2007-10-14 |
| 基金项目:国家863重点资助项目（2006AA100101）；中国博士后基金资助项目(2005037696)；湖南省级重点实验室科研项目(05FJ4035) |
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| Isolation and Characterization of NBS-LRR Resistance Gene Homology Sequences from Dongxiang Wild Rice |
| WANG Zhenghua1, GAO Xiujie1, CAO Mengliang2 |
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(1.College of Computer, National Univ. of Defense Technology, Changsha 410073，China;2.National Hybrid Rice R&D Center，Changsha 410125，China)
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| Abstract: |
| The resistant gene of plants has conservative sequence. Probes were designed in terms of the sequences of known resistance gene. With the colony hybridization method, the genomic library of Dongxiang wild rice was screened and two candidate clones were obtained. The sequences were analyzed with the blast program to the GenBank database and a conservative NB-ARC domain was found in both of the sequences. The clones would be transferred into rice for a further analysis. |
| Keywords: NBS-LRR resistance gene colony hybridization genomic library |
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